膀胱癌与P16基因缺失关系的探讨

e P16 gene was analysi s by duplex PCR in 31 bladder cancer tissue samples.Results:The gene deletions were found in seven samples.The deletion ratio of P16 gene was 22.6 per cent.Five cases had deletion in exon 2. One case had deletion in exon 1 and one had deletion in both exon 1 and exon 2.Conclusions:The results suggest that the deletion of P16 gene were associated with some bladder cancer.

　　Key words　Bladder tumor　Gene,P16　Polymeruse chain reaction　Gene deletion

　　1993年，Serrano等〔1〕在研究与CDK4作用的蛋白时发现了P16基因，并克隆了P16的cDNA。随后，许多学者在不同的肿瘤细胞中相继发现了P16基因的缺失或突变。这些结果提示P16基因的失活与肿瘤的发生有关。膀胱癌中P16基因的存在情况国内外报道很少。为了探讨膀胱癌的发生与P16基因缺失的关系，本研究采用双重PCR法对31例膀胱癌病理组织中P16基因进行了检测，现将结果报告如下。

　　1　材料与方法

　　1.1　标本制备

　　选取我院手术切除的膀胱癌新鲜标本31例，每例标本一部分立即冷冻保存，一部分作常规石蜡切片。另取5例远离癌肿的正常膀胱粘膜组织作正常对照。石蜡标本均制成厚约5 μm的连续组织切片，进行H-E染色和病理组织学检查，病理检查诊断为膀胱移行细胞癌。按Ash病理分级〔2〕和UICC临床分期〔3〕对肿瘤细胞分化程度和浸润范围进行评估。

　　1.2　DNA制备及定量

　　参照文献〔4〕的方法制备DNA，用紫外分光光度计进行DNA定量。

　　1.3　双重PCR扩增

　　根据GenBank所给P16的核苷酸序列，设计

 
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